Research Area Plant organs express unique and overlapping sets of mRNAs, such differential gene expression provides the bases for plant development and specialization of function. A productive approach to a molecular investigation of plant development has been the isolation and characterization of organ- and tissue-specific genes. My research is aimed at understanding the molecular mechanism involved in root development. Root development involves strategies that are common to the development of all plant organs, as well as certain aspects that are unique to roots. We have isolated a number of root-specific cDNA clones from bean seedling root cDNA library by differential screening. Clues to biological role of predicted protein are available from study of gene expression pattern and protein localization by in situ hybridization and immunohistochemical analysis. We are also interested in the molecular mechanism involved in cell division during petal protoplast culture of petunia. Several proteins involved in the first cell division have been investigated by two-dimensional SDS-PAGE. Genes involved in the cell division are also being screened and characterized by Expression Sequence Tagging(EST) procedure
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